The antibody-based pharmaceuticals resource library’s Podcast

Yeast display is a novel technique widely used to express the proteins at the yeast surface after translation and maturation in a eukaryotic system. This episode gives a comprehensive introduction to yeast display, including yeast display system, the process of yeast display system construction, and application of yeast display. 

In this episode, we will introduce four aspects of ribosome display, including introduction to ribosome display technology, principle of ribosome display, it's system, and advantages and applications of ribosome display.

Previously, we published part one of recombinant antibody overview which mainly focuses on antibody basic information and antibody production in vivo. In part two of this topic, we will give an introduction to recombinant antibody and recombinant antibody expression.

In this episode, we will give you a whole understanding of conventional antibody, recombinant antibody, and their basic information and expression.

In this episode, we will introduce different antibody expression systems, including common process of recombinant antibody expression and generalized features of antibody in various expression systems.

Recombinant antibodies are antibody fragments generated using recombinant antibody coding genes as a source and display technology, delivering high reproducibility, specificity, and scalability. This episode provides a comprehensive introduction to recombinant antibodies, including background, principle, and production process. 

In this episode, Creative Biolabs will introduce technologies of monoclonal antibody development, including hybridoma, surface display, B cell immortalization, and transgenetic technology. You will have a whole concept of mAb development after listening to the episode. 

What is de novo antibody sequencing? What are the steps of de novo antibody sequencing?  Are there any challenges in de novo antibody sequencing? How do we overcome these difficulties in antibody sequence analysis? Creative Biolabs will describe all these issues in the episode. 
 

Bacterial display is a protein engineering technique widely used for in vitro protein evolution. In this episode, many aspects of bacterial display will be illustrated, such as cell surface display system in gram-positive/negative bacteria, components of bacterial display systems, construction and screening process of bacterial display library, and application of bacterial display.

As a new preparation technology for genetically engineered antibodies, antibody library technology has important theoretical and practical values in the cloning of human antibodies, antibody performance modification, and antibody gene research. Here, we will explore the de novo antibody library screening technologies and corresponding applications.